Regarding our work on roots, the method we currently use in chickpea,
ie, root extraction from plants grown for about 35 days in 1.2
m length and 16 cm diameter, only provides a “static” measurement
of root growth, and cannot capture the highly dynamic growth of
roots, which is largely influenced by environmental factors, in
particular water deficits. The current system does not inform
on the volume of water uptake, neither on the kinetics of water
uptake, which we feel are extremely important to properly understand
the contribution of roots to drought tolerance. Therefore, we
have developed a lysimetric system to allow the direct measurement
of water uptake in-vivo. In the first picture, we can
see the cylinder design, with a collar attachment allowing its
lifting for weighing. In the second picture, we can see that the
weighing procedure, carried out with a hanging scale, requires
minimum labor and effort (cylinder is lifted with a block chain
hoist), and this should also improve the throughput in root studies.
The system shown in the pictures is the one used in P2 facilities
to analyze the effect of a water deficit on water uptake response
of rd29::DREB1A transgenic groundnut events. A large-scale facility
to test these effects on a large range of germplasm of ICRISAT's
mandate crops is being developed with funding from BDT (Department
of BioTechnology, Government of India), in the framework of the
recently started CEG (Center of Excellence in Genomics) project.
For further information, contact:
Vincent Vadez |