Plant Physiology Root Research Methods


Regarding our work on roots, the method we currently use in chickpea, ie, root extraction from plants grown for about 35 days in 1.2 m length and 16 cm diameter, only provides a “static” measurement of root growth, and cannot capture the highly dynamic growth of roots, which is largely influenced by environmental factors, in particular water deficits. The current system does not inform on the volume of water uptake, neither on the kinetics of water uptake, which we feel are extremely important to properly understand the contribution of roots to drought tolerance. Therefore, we have developed a lysimetric system to allow the direct measurement of water uptake in-vivo. In the first picture, we can see the cylinder design, with a collar attachment allowing its lifting for weighing. In the second picture, we can see that the weighing procedure, carried out with a hanging scale, requires minimum labor and effort (cylinder is lifted with a block chain hoist), and this should also improve the throughput in root studies. The system shown in the pictures is the one used in P2 facilities to analyze the effect of a water deficit on water uptake response of rd29::DREB1A transgenic groundnut events. A large-scale facility to test these effects on a large range of germplasm of ICRISAT's mandate crops is being developed with funding from BDT (Department of BioTechnology, Government of India), in the framework of the recently started CEG (Center of Excellence in Genomics) project.

 

For further information, contact: Vincent Vadez