BACs and BESs

 

            A bacterial artificial chromosome (BAC) library has been produced at UC-Davis for the PGI reference genotype-“Asha”. The library was constructed based on partial digestion of high molecular weight DNA separately with HindIII and BamHI restriction enzymes. The HindIII library is composed of 34,560 clones with an estimated average insert size of 120,000 bp, while the BamHI library is composed of 34,560 clones with an estimated average insert size of 115 kb. Taken together, the combined clones represent ~11X coverage of the pigeonpea genome.

            A set of randomly selected 50,000 BAC clones was subjected to end sequencing by UC-Davis and ICRISAT in collaboration with JCVI, USA. In total, 87,590 BAC end sequences (BESs) were generated, with most of the 50,000 BAC clones containing high quality sequence from both ends. The combined data represent ~56 Mbp of DNA sequences, which were submitted to the National Center for Biotechnology Information (NCBI) Genome Survey Sequence (GSS) database for public access.

Download BAC-end Sequence Dataset

     Full Set - 84,927 entries

     Non-redundant set - 39,945 entries

(based on CAP3 assembly of full set)