SSR

 

             About 7000 SSR markers have been designed, validation has been completed for about 3000 markers. Analysis is in progress.

Advances in development of SSR markers in pigeonpea

Features SSR enriched library BAC-end sequences ESTs Total
Sanger sequencing 454 Sequencing
ICRISAT NRCPB
Number of clones 3,072 50,000 10,376
Sequence surveyed 82 87,590 5,085 188,741 1,696,724
Amount of sequence data (kb) 60.8 56,506.90 2,878.30 30,876.16 56,600.00
SSRs identified 36 18,149 3,583 87,314 130,850
SSRs frequency (in kb) 1/1.68 1/3.11 1/0.80 1/0.35
Primer pairs designed 23 6,590 383 In progress 6,996
Primer pairs synthesized 23 3,072 84 3,179
Primers amplified 16 2,565 52 400 3,033

            Primer Sequence for some SSR Markers are available in Saxena et al. 2010, Odeny et al. 2007 & Odeny et al 2009.

Primer sequence will be available soon.