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Hands-on training program on Gene Editing in Plants using CRISPR/Cas9 System
December 19, 2022 @ 8:00 am - December 23, 2022 @ 5:00 pm
Last date: 12th December 2022
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Course description
The new breeding technique (NBT), gene editing, which enables very rapid and precise trait production and selection without having any negative effects on the native phenotypes, lies at the heart of the newage agricultural breakthroughs currently available to breeders. These techniques will also speed up genetic advancements and product delivery, enabling “disruptive reductions” in the input costs for farmers and “exponential improvements” in nutrition and production.
Gene editing technologies have revolutionized the ability to alter DNA at the molecular level, and this technology has implications that go well beyond those of conventional molecular biology. A hands-on training program on “Gene Editing in plants using CRISPR/Cas9 system” is a comprehensive training program ideal for researchers who are looking for a balanced theoretical and hands-on introduction to gene editing. The training program includes both theory and hands on practical for better understanding the various components and stages of gene editing.
The training will help to learn the basic gene editing workflows, from design and synthesis of target-specific guide RNAs (gRNAs), delivery of gRNAs in plant cells, detection, and analysis of gene editing efficiencies. This is a great opportunity to interact and benefit from the expertise of our scientists and collaborators, as they help you design your first gene editing experiment. We are confident that by the end of the workshop, participants will be able to develop their experimental workflows.
Objective
- Cover the basic concept of gene editing technology
- Bioinformatic tools for design guide-RNA and CRISPR/Cas9 construct
- Train participants in high-throughput techniques for gene editing application
- Introduction to functional validation of edited lines
- Understand the ethics and biosafety of gene editing technologies
BioNcube is a BIRAC-Bio incubator supporting Ag-biotech innovation, development, and applications of broad range of biotechnological solutions spreading across various domains from basic research to product translation. Agribiotech start-ups incubated in BioNcube, have access to the scientific knowledge of ICRISAT, biotechnology laboratories with state-of- the-art equipments and infrastructure such as plant genotyping, phenotyping and transgenic facilities, greenhouses, plug-and- play modular labs, molecular biology lab, analytical lab, transformation facility, contained fields etc.
The value proposition of the BIRAC- Bio incubator is to link business incubation to translation and support ag-biotech start-ups from proof-of- concept stage through to technology translation and commercialization that will further benefit farming communities.
Lectures
- Editing technologies applications and future prospective
- CRISPR/Cas9 based gene editing tools for stress tolerance in crop plants
- Editing rice-genome with CRISPR/Cas9: to improve agronomic traits for increased productivity
- New breeding tools for accelerated breeding in dryland cereals
Practical sessions
- Bioinformatics tools for gene editing
- Strategy and cloning of the guide RNA (s) and Cas9 into the plant expression vectors
- Gateway cloning for the CRISPR/Cas9 vectors
- Agrobacterium transformation for the CRISPR/ Cas9 recombinant plasmids by electroporation
- Complete demonstration on development of the gene-edited plants through Agrobacterium-mediated transformation
- Molecular analysis of the gene-edited plants
- InDels identification and analysis
Resource persons/trainers
Resource persons for this course would be from National and International Research organizations.
Course fees
- Students/Postdoc
INR 14,000 (without accommodation)
INR 25,000 (with accommodation) - Scientist/Faculty
INR 17,000 (without accommodation)
INR 28,000 (with accommodation) - Industry
INR 30,000 (without accommodation)
INR 45,000 (with accommodation)
Course language
All course notes and lectures will be in English. Therefore, participants should have a good knowledge of English and of the appropriate technical terms of Gene editing technology.
Venue
The venue for the training program is Platform for Translational Research on Transgenic Crops (PTTC) building, ICRISAT Campus, Patancheru, Hyderabad.
Accommodation
The participants will be accommodated in the Guest House/Hotel during the course of the training. The cost of any additional stay (beyond the dates of training) would be at trainees own expense. Information on extended stay needs to be given in advance.
More information
Additional information on the course will be provided to all the participants who are selected for admission to the course.
Applications are invited from researchers who are familiar with basic molecular and cell biology techniques and want to learn genome editing applications in agriculture using the most recent and advanced CRISPR systems. While previous experience in genome editing is not required, it is expected that the participants have fundamental knowledge and working experience on molecular biology and transformation tools. The application can be accessed from the following link or format provided in the brochure.
https://forms.gle/kgHvsTAZJsZH2LQ49
The completed application should be submitted through the link or sent to bioncube@cgiar.org with copy to wricha.tyagi@cgiar.org, P.Sudhakarreddy@cgiar.org and k.yogendra@cgiar.org.
The due date for application is 12 December 2022