- This event has passed.
Hands-on training program on Mass Spectrometry-based Proteomics and Metabolomics
November 21, 2022 @ 8:00 am - November 25, 2022 @ 5:00 pm
BioNcube, a BIRAC-Bio incubator of International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) call applications for a hands-on training program on “Mass Spectrometry Based Proteomics and Metabolomics”. ICRISAT is an International non-profit agricultural research institute with stateof- the-art facilities for Agri-biotechnology research and product translation. The training course is being organized from November 21-25th at ICRISAT, Patancheru, Hyderabad, India-502 324
Course description
Over the last few decades, mass spectrometry has developed into a powerful tool that is used in a variety of ways across numerous disciplines. With the aid of these technologies, we can gain a deeper understanding of plant biochemistry, which leads to important advancements. These include identifying phytochemicals with nutritional and medicinal properties, detecting biomarkers for genetic variation identification, and understanding plant defense mechanisms. Mass spectrometric instruments are used in proteomics and metabolomics to measure, characterize, and quantify proteins and metabolites. In conjunction with this technology, it is now possible to enhance the nutritional quality of crop plants by understanding and manipulating targeted proteins and metabolites.
In recent years, mass spectrometry-based proteomics and metabolomics have emerged as powerful tools for identifying, characterizing, and quantifying proteins and metabolites important for cellular function. The purpose of this training program is to introduce proteomics and metabolomics principles, both theoretically and practically, to assist participants in better application of the principles.
This course will provide you with a basic understanding of mass spectrometry, sample preparation, data analysis and application(s). It is a good opportunity to gain a deeper understanding of proteomics and metabolomics studies in cells. Additionally, at the end of the program you will have the opportunity to interact with our scientists and experts to gain a better understanding of the course. It is our hope that, at the completion of this program, all the participants will be able to apply for the research areas they are interested in and develop their own workflows based on this knowledge.
Objective
- A brief introduction to basic chromatography and mass spectrometry
- The basics of proteomics and metabolomics
- Procedural steps for the extraction of proteins and metabolites
- Sample preparation and processing for Liquid chromatography mass spectroscopy
- Data analysis and interpretation
Lectures
- Basic principles of mass spectrometry as well as liquid chromatography
- Introduction to Metabolomics workflow specifically non-targeted metabolomics
- Introduction to basic proteomics
- Quantitative proteomics strategies such as label free quantification
- Integration of metabolomics and proteomics approaches to identify candidate genes responsible for biotic stresses
- Intellectual property issues in access and sharing data.
Practical sessions
- Protein extraction and quantification
- SDS-PAGE and gel staining
- 2D-Gel electrophoresis
- In gel and in-solution digestion
- HPLC demonstration
- Metabolite extraction
- Sample preparation for LCMS
- Data analysis and interpretation
- Intellectual property.
Resource persons/trainers
Resource persons for this course would be from National and International Research organizations.
Course fees
- Students/Postdoc INR 20,000 (without accommodation) INR 35,000 (with accommodation)
- Scientist/Faculty INR 25,000 (without accommodation) INR 40,000 (with accommodation)
- Industry INR 50,000 (with accommodation)
Course language
All course notes and lectures will be in English. Therefore, participants should have a good knowledge of English and of the appropriate technical terms of metabolomics and proteomics technology.
Venue
The venue for the training program is Platform for Translational Research on Transgenic Crops (PTTC) building, ICRISAT Campus, Patancheru, Hyderabad.
Accommodation
The participants will be accommodated in the Guest House/Hotel during the course of the training. The cost of any additional stay (beyond the dates of training) would be at trainees own expense. Information on extended stay needs to be given in advance.
More information
Additional information on the course will be provided to all the participants who are selected for admission to the course.
BioNcube is a BIRAC-Bio incubator supporting Ag-biotech innovation, development, and applications of broad range of biotechnological solutions spreading across various domains from basic research to product translation. Agribiotech start-ups incubated in BioNcube, have access to the scientific knowledge of ICRISAT, biotechnology laboratories with state-of- the-art equipments including LC-MS, HPLC, 2-D apparatus and SDS-PAGE, and infrastructure such as plant genotyping, phenotyping and transgenic facilities, glasshouses, greenhouses, molecular biology lab, transformation facility, contained fields, etc. The value proposition of the BIRAC- Bio incubator is to link business incubation to translation and support ag-biotech start-ups from proof-of-concept stage through to technology translation and commercialization that will further benefit farming communities.
Applications are invited from researchers who are familiar with basic molecular and cell biology techniques and want to learn proteomics and metabolomics applications in agriculture using the most recent and advanced Mass spectrometry systems. While previous experience in this technology is not required, it is expected that the participants have fundamental knowledge and working experience on chromatography. The application can be accessed from the following link format provided in the brochure.
https://forms.office.com/r/k67GbryAhF
The completed application should be submitted through the link or sent to bioncube@cgiar.org with copy to wricha.tyagi@cgiar.org; k.yogendra@cgiar.org, and P.Sudhakarreddy@cgiar.org.
The due date for application is 04 November 2022